StressCovidV1, Main, Exploration, bibRecord, 001126

Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein

Identifieur interne : 001126 ( Main/Exploration ); précédent : 001125; suivant : 001127

Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein

Auteurs : Tsuey-Ching Yang [Taïwan] ; Shih-Wein Li [Taïwan] ; Chien-Chen Lai [Taïwan] ; Kai-Zen Lu [Taïwan] ; Man-Tzu Chiu [Taïwan] ; Tsung-Han Hsieh [Taïwan] ; Lei Wan [Taïwan] ; Cheng-Wen Lin [Taïwan]

Source :

PROTEOMICS [ 1615-9853 ] ; 2013-12.

RBID : ISTEX:C525F44FD0071E5F1174A64F2AF826E07EC4D412

English descriptors

Teeft :
- Antagonistic ability, Antagonistic activity, Antagonistic function, Apoptosis, Assay, Calcineurin, Calcineurin inhibitor, Calcineurin sirna, Calreticulin, Calreticulin downregulation, Calreticulin silence, Calreticulin sirna, Cell monolayers, Cell types, Cells transfected, Chaperone, Control sirna, Cyclophilin, Downregulation, Empty vector, Encephalitis, Gapdh mrna, Gene expression, Gene silence, Gmbh, Human medulloblastoma cells, Inhibitor, Interferon, Intracellular, Japanese encephalitis, Japanese encephalitis virus, Kgaa, Localization, Luciferase, Lysates, Medulloblastoma, Mrna, Mrna expression, Mrna levels, Ns5expressing cells, Nuclear localization, Nuclear translocalization, Nuclear translocation, Pathway, Phosphorylation, Plaque titers, Post infection, Promoter, Promoter activity, Protease, Proteomic analysis, Proteomics, Relative ratio, Renilla luciferase, Replication, Single treatment, Sirna, Stat1, Stat1 phosphorylation, Subcellular, Subcellular localization, Subcellular location, Thioredoxin, Transfected, Translocation, Tyrosine phosphorylation, Upregulated, Vector control, Vector control cells, Vector controls, Verlag, Verlag gmbh, Viral, Viral replication, Virol, Weinheim, Western blot.

Abstract

Japanese encephalitis virus (JEV) nonstructural protein 5 (NS5) exhibits a Type I interferon (IFN) antagonistic function. This study characterizes Type I IFN antagonism mechanism of NS5 protein, using proteomic approach. In human neuroblastoma cells, NS5 expression would suppress IFNβ‐induced responses, for example, expression of IFN‐stimulated genes PKR and OAS as well as STAT1 nuclear translocation and phosphorylation. Proteomic analysis showed JEV NS5 downregulating calreticulin, while upregulating cyclophilin A, HSP 60 and stress‐induced‐phosphoprotein 1. Gene silence of calreticulin raised intracellular Ca2+ levels while inhibiting nuclear translocalization of STAT1 and NFAT‐1 in response to IFNβ, thus, indicating calreticulin downregulation linked with Type I IFN antagonism of JEV NS5 via activation of Ca2+/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5‐mediated inhibition of IFNβ‐induced responses, for example, IFN‐sensitive response element driven luciferase, STAT1‐dependent PKR mRNA expression, as well as phosphorylation and nuclear translocation of STAT1. Transfection with calcineurin (vs. control) siRNA enhanced nuclear translocalization of STAT1 and upregulated PKR expression in NS5‐expressing cells in response to IFNβ. Results prove Ca2+, calreticulin, and calcineurin involvement in STAT1‐mediated signaling as well as a key role of JEV NS5 in Type I IFN antagonism. This study offers insights into the molecular mechanism of Type I interferon antagonism by JEV NS5.

Url:

DOI: 10.1002/pmic.201300001

Affiliations:

Taïwan

Le document en format XML

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<front><div type="abstract">Japanese encephalitis virus (JEV) nonstructural protein 5 (NS5) exhibits a Type I interferon (IFN) antagonistic function. This study characterizes Type I IFN antagonism mechanism of NS5 protein, using proteomic approach. In human neuroblastoma cells, NS5 expression would suppress IFNβ‐induced responses, for example, expression of IFN‐stimulated genes PKR and OAS as well as STAT1 nuclear translocation and phosphorylation. Proteomic analysis showed JEV NS5 downregulating calreticulin, while upregulating cyclophilin A, HSP 60 and stress‐induced‐phosphoprotein 1. Gene silence of calreticulin raised intracellular Ca2+ levels while inhibiting nuclear translocalization of STAT1 and NFAT‐1 in response to IFNβ, thus, indicating calreticulin downregulation linked with Type I IFN antagonism of JEV NS5 via activation of Ca2+/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5‐mediated inhibition of IFNβ‐induced responses, for example, IFN‐sensitive response element driven luciferase, STAT1‐dependent PKR mRNA expression, as well as phosphorylation and nuclear translocation of STAT1. Transfection with calcineurin (vs. control) siRNA enhanced nuclear translocalization of STAT1 and upregulated PKR expression in NS5‐expressing cells in response to IFNβ. Results prove Ca2+, calreticulin, and calcineurin involvement in STAT1‐mediated signaling as well as a key role of JEV NS5 in Type I IFN antagonism. This study offers insights into the molecular mechanism of Type I interferon antagonism by JEV NS5.</div>
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Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein

Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein

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English descriptors

Abstract

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